法国euromedex细菌双杂交系统试剂盒EUK001
法国euromedex细菌双杂交系统试剂盒EUK001
细菌双杂交系统试剂盒（腺苷酸环化酶）
( 英文名称：BACTH System Kit)
细菌双杂交系统试剂盒（腺苷酸环化酶）EUK001
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Designation :
BACTH System Kit
Size :
1 kit
Informations scientifiques :
The BACTH system has been developed by the group of Dr. D. Ladant at the Pasteur Institute and is based on the interaction-mediated reconstitution of the adenylate cyclase activity in E. coli (1). It exploits the fact that the catalytic domain of adenylate cyclase (CyaA) from Bordetella pertussis (2) consists of two complementary fragments, T25 and T18 (Figure 1A), that are not active when physically separated (Figure 1B). When these two fragments are fused to interacting polypeptides, X and Y, heterodimerization of these hybrid proteins results in functional complementation between T25 and T18 fragments and, therefore, cAMP synthesis (Figure 1C). Cyclic AMP produced by the reconstituted chimeric enzyme binds to the catabolite activator protein, CAP. The cAMP/CAP complex is a pleiotropic regulator of gene transcription in E. coli. It turns on the expression of several resident genes, including genes of the lac and mal operons involved in lactose and maltose catabolism (Figure 1D). Therefore, bacteria become able to utilize lactose or maltose as the unique carbon source and can be easily distinguished on indicator or selective media.
Type :
Double hybride (Bacteria)
Designation :
BACTH System Kit
Size :
1 kit
Informations scientifiques :
The BACTH system has been developed by the group of Dr. D. Ladant at the Pasteur Institute and is based on the interaction-mediated reconstitution of the adenylate cyclase activity in E. coli (1). It exploits the fact that the catalytic domain of adenylate cyclase (CyaA) from Bordetella pertussis (2) consists of two complementary fragments, T25 and T18 (Figure 1A), that are not active when physically separated (Figure 1B). When these two fragments are fused to interacting polypeptides, X and Y, heterodimerization of these hybrid proteins results in functional complementation between T25 and T18 fragments and, therefore, cAMP synthesis (Figure 1C). Cyclic AMP produced by the reconstituted chimeric enzyme binds to the catabolite activator protein, CAP. The cAMP/CAP complex is a pleiotropic regulator of gene transcription in E. coli. It turns on the expression of several resident genes, including genes of the lac and mal operons involved in lactose and maltose catabolism (Figure 1D). Therefore, bacteria become able to utilize lactose or maltose as the unique carbon source and can be easily distinguished on indicator or selective media.
Type :
Double hybride (Bacteria)
Category :
N/A
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